霍方俊，男，博士，1978年12月生，有机化学教授，硕士生导师。主持国家自然科学基金1项、山西省自然科学基金2项，参与5项国家基金和多项山西省科研项目，获得授权发明专利10项，在Chem Soc Rev，J Am Chem Soc，Org Lett，Anal Chem，Sci Rep，Analyst，Sens Actuators B，Dyes Pigments等期刊发表论文150余篇，获得山西省自然科学三等奖1项，2018年度入选山西省“三晋英才”支持计划青年优秀人才。
研究方向
荧光探针及其生物传感。
主持或参加科研项目
1、山西省自然科学基金，201901D111015，光激活型多反应位点/癌细胞靶向二氧化硫精准荧光检测，2019/12-2022/12，5万元，主持；
2、国家自然科学基金面上项目，21775096，去甲肾上腺素（NE）、多巴胺（DA）、谷氨酸（Glu）神经递质的特异 性荧光探针设计及其神经成像，2018/1 -2021/12，64万元，第二参与人；
3、国家自然科学基金青年项目，21705102，可同时检测过氧化氢和癌细胞侵袭转移风险因子的发光体系的构建及其生物成像研究，2018/1-2020/12，24万元，第二参与人；
4、国家自然科学基金面上项目，21472118， 用于区分Cys,Hcy和GSH的新型的超分子漏斗筛和多结合位点的探针及其在蛋白标记的应用，2015/1-2016/12，50万元，第二参与人；
5、国家自然科学基金青年项目，21102086，基于"硫醇-色烯"关环反应的可再生化学计量器属于重金离子检测和生物成像的研究，2012/01-2014/12，25万元，主持；
6、山西省自然科学基金，2012021009-4，基于点击反应的硫醇小分子光学探针的研究，2012/01-2014/12，3万元，主持；
7、国家自然科学基金面上项目，21072119，基于硫醇-色烯‘点击’开环反应的硫醇小分子光学探针设计及其反应机制研究，2011/01-2013/12，36万元，第二参与者；
8、 国家自然科学基金青年项目，20801032，溶液中阴离子识别及其传感器的设计研究，2009/01-2011/12，18万元，第二参与者。
代表性论文
1. Weijie Zhang#, Fangjun Huo#, Fangqin Cheng, Caixia Yin*. Employing an ICT-FRET integration platform for the real-time tracking of SO2 metabolism in cancer cells and tumor models, J. Am. Chem. Soc., 2020, 142, 6324-6331.
2. Weijie Zhang#, Fangjun Huo#, Yongkang Yue, Yongbin Zhang, Jianbin Chao, Fangqin Cheng, Caixia Yin*. Heat stroke in cell tissues related to sulfur dioxide level is precisely monitored by light-controlled fluorescent probes, J. Am. Chem. Soc., 2020, 142, 3262-3268.
3. Fangjun Huo, QingWu, Caixia Yin,Weijie Zhang, Yongbin Zhang. A high efficient and lysosome targeted “off-on” probe for sulfite based on nucleophilic addition and ESIPT, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019, 214, 429-435.
4. Yongkang Yue, Fangjun Huo, Fangqin Cheng, Xijing Zhu, Tendai Mafireyi, Robert M. Strongin*, Caixia Yin*. Functional synthetic probes for selective targeting and multi-analyte detection and imaging, Chem. Soc. Rev., 2019, 48, 4155-4177.
5. Fangjun Huo, Qing Wu, Jin Kang, Yongbin Zhang, Caixia Yin∗. A specific fluorescent probe for zinc ion based on thymolphthalein and it’s application in living cells, Sensors and Actuators B: Chemical, 2018, 262, 263–269.
6. Fangjun Huo, Yaqiong Zhang, Peng Ning, Xiangming Meng, Caixia Yin. A novel isophorone-based red-emitting fluorescent probe for selective detection of sulfide anions in water for in vivo imaging, J. Mater. Chem. B, 2017, 5, 2798-2803.
7. Yongkang Yue#, Fangjun Huo#, Peng Ning, Yongbin Zhang, Jianbin Chao, Xiangming Meng*, Caixia Yin*. Dual-Site Fluorescent Probe for Visualizing the Metabolism of Cys in Living Cells, J. Am. Chem. Soc., 2017, 139, 3181−3185.
8. Fangjun Huo, Jin Kang, Caixia Yin, Jianbin Chao, Yongbin zhang. Highly selective fluorescent and colorimetric probe for live-cell monitoring of sulphide based on bioorthogonalreaction, Scientific Reports, 2015, 5, 8969.
9. Fangjun Huo, Jin Kang, Caixia Yin, Jianbin Chao, Yongbin Zhang. A turn on fluorescent sensor for cyanide based on ICT off in aqueous and its application for bioimaging, Sensors and Actuators B: Chemical, 2015, 215, 93-98.
10. Fangjun Huo, Jin Kang, Caixia Yin, Jianbin Chao, Yongbin Zhang. A turn-on green fluorescent thiol probe based on the 1,2-additionreaction and its application for bioimaging, Sensors and Actuators B: Chemical, 2015, 207, 139-143.
11. Caixia Yin, Jingjing Zhang, Ramon Martinez-Manez*, Fangjun Huo*, Yutao Yang, Haigang Lv, Sidian Li*. Thiol-addition reactions and their applications in thiol recognition, Chemical Society Reviews, 2013, 42(14), 6032-6059.
12. Fangjun Huo, Yutao Yang, Caixia Yin, Yueyin Chu, Jianbin Chao, Yongbin Zhang, Jingjing Zhang, Sidian Li, Haigang Lv, Anmin Zheng, Diansheng Liu. Combined spectral experiment and theoretical calculation to study the chemosensors of copper and their applications in anion bioimaging, Sensors and Actuators B: Chemical, 2013, 177, 1189-1197.
13. Fangjun Huo, Long Wang, Caixia Yin, Yutao Yang, Hongbo Tong, Jianbin Chao, Yongbin zhang. The synthesis, characterization of three isomers of rhodamine- derivative and their application in copper (II) ion recognition, Sensors and Actuators B: Chemical, 2013, 188, 735-740.
14. Fangjun Huo, Caixia Yin, Yutao Yang, Jing Su, Jianbin Chao, Diansheng Liu. Ultraviolet-Visible Light (UV−Vis)-Reversible but Fluorescence-Irreversible Chemosensor for Copper in Water and Its Application in Living Cells, Analytical Chemistry, 2012, 84, 2219-2223.
15. Fangjun Huo, Jingjing Zhang, Yutao Yang, Jianbin Chao, Caixia Yin, Yongbin Zhang, Tinggui Chen. A fluorescein-based highly specific colorimetric and fluorescent probe for hypochlorites in aqueous solution and its application in tap water, Sensors and Actuators B: Chemical, 2012, 166-67, 44-49.
16. Fangjun Huo, Yutao Yang, Jing Su, Yuanqiang Sun, Caixia Yin, Xuxiu Yan. Indicator approach to develop a chemosensor for the colorimetric sensing of thiol-containing water and its application for the thiol detection in plasma, Analyst, 2011, 136(9), 1892-1897.
17. Fangjun Huo, Yuanqiang Sun, Jing Su, Yutao Yang, Caixia Yin, Jianbin Chao. Chromene "Lock", Thiol "Key", and Mercury(II) Ion "Hand": A Single Molecular Machine Recognition System, Organic Letters, 2010, 12(21), 4756-4759.
18. Fangjun Huo, Yuanqiang Sun, Jing Su, Jianbin Chao, Haijuan Zhi, Caixia Yin. Colorimetric Detection of Thiols Using a Chromene Molecule, Organic Letters, 2009, 11(21), 4918-4921.
授权发明专利
1、霍方俊，周娜，阴彩霞，一种异恶唑衍生物及其合成方法和检测硫化氢的应用，2020.4.17，中国，ZL201811324214.9
2、霍方俊，阴彩霞，一种CN—检测试剂及其合成方法和应用，2018.10.16，中国，ZL201710003440.6
3、霍方俊，阴彩霞，一种1,2-苯并异噻唑-3-酮的制备方法，2018.1.2，中国，ZL 201610255264.0
4、霍方俊，阴彩霞，一种试剂及其在硫醇检测中的应用，2016.2.10，中国，ZL201410194221.7
5、霍方俊，阴彩霞，一种检测硫醇的试剂和方法，2015.7.29，中国，ZL201310488140.3
6、霍方俊，阴彩霞，一种试剂及其在硫化氢检测中的应用，2015.6.3，中国，ZL201410194129.0。
7、霍方俊，阴彩霞，杨瑜涛，张改清，一种检测同型半胱氨酸的试剂和方法，2013.1.23，中国，ZL201110191582.2
8、霍方俊，刘艳玲，阴彩霞，孙远强，快速检测水溶液中无机磷的方法，2011.1.5，中国，ZL200910073643.8
9、霍方俊，阴彩霞，孙远强，快速检测水溶液中半胱氨酸的方法，2010.6.23，中国，ZL200810055453.9
10、霍方俊，阴彩霞，杨频，一种基于胍环的核算水解切割试剂，2008.7.30，中国，ZL200610048393.9
联系方式
地址：山西省太原市坞城路92号，山西大学应用化学研究所
邮编：030006
邮箱：huofj@sxu.edu.cn
